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KMID : 0380020030180020149
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 2 p.149 ~ p.154
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Isolation and Characterization of Hydrogen Producing Bacterium
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Lee Ki-Seok
Kang Chang-Min
Jung Sun-Yong
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Abstract
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The hydrogen-producing bacterium was isolated from fresh water and identified as Enterobacter cloacae. The isolated was named Enterobacter cloacae YJ-1. In batch culture, The optimum cultivation temperature and pH of strain YJ-1 was 35℃ and 7.5, respectively. All of the added glucose was consumed completely during fermentation even though pH was not controlled. Amount of hydrogen produced on each condition of 2% glucose, 4% sucrose and 5% fructose was 950, 1000 and 948 mL/L, respectively and resulted in increasing hydrogen production approximately 2.5-times more than controlled condition. The maximum hydrogen production was obtained when 50 mM phosphate was added. In repeated-batch culture, hydrogen gas of 1920 mL/L was totally produced for 48. The maximum hydrogen was produced on the condition of 0.5% yeast extract, but the production amount was not changed on the condition of over 0.5%. Most of the organic acids produced during the fermentation were formic and acetic acid, and propionic acid was moiety also generated.
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KEYWORD
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Hydrogen production, Enterobater cloacae, organic acids
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